Page 31 - CIBEREHD-2015-eng

Proteomics Platform
In 2015 the proteomics platform provided service for, and cooperated with, different groups in the CIBEREHD (bioGUNE, H. Clinic, ITGP and BioDonostia). Six articles were published in scientific journals with international impact. Methods for preparing the sample and acquisition for what is known as peptidomics were also made ready. Lastly, the work done in preparing the differential quantification of proteomes by the nanoscale liquid chromatography method coupled on line
Platforms
Metabolomics Platform (RMN)
In 2015, the NMR metabolomics platform consol- idated and validated the standardised work proce- dures for metabolomic analysis of urine and serum. More specifically, the protocols for preparing sam- ples and acquisition of spectra were adapted to the ones established by the company Bruker and the international consortium led by the Imperial College and the I.T. solutions required for univariate and multivariate analysis of the sets of data were imple- mented.
to tandem mass spectrometers by the label-free approach (Label-Free nLC MS/MS Quantification), was reflected in the publication of the cooperation performed with Dr Woddhoo (Gómez-Sánchez et al. J Cell Biol).
In the field of applications, a set of samples of serum from patients with hepatic fibrosis was analysed. Metabolomic analysis by NMR has enabled estab- lishing a protocol for separation of stages 1 and 4 of hepatic fibrosis and has identified metabolites able to act as fibrotic progression markers. Said results are shown in a scientific publication (currently being revised).
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