Page 30 - CIBEREHD-2015-eng

Platforms
CIBERHEP. Platform for Chronic Hepatitis B
The CIBERHEP platform for chronic hepatitis B is a cooperation scheme run by the CIBER and the Asociación Española para el Estudio del Hígado (AEEH). At the present time this is the main data- base of patients being treated for chronic hepatitis B in Spain: in 2015 data was registered on around 1300 patients monitored at 25 centres in 9 different administrative regions.
This data has meant that different studies could be carried out, such as the analysis of the effectiveness of entecavir and tenofovir in patients previously treated at the clinical practice, presented as a poster at the 40th annual congress of AEEH (Madrid, 24- 27th February 2015). A study has also been made on the impact of nucleoside/nucleotide analogue antivirals in the incidence of hepatocellular carcino-
ma in Caucasian patients, the results of which were recently presented as an oral communication at the 41st Annual Congress of the AEEH (Madrid, 17-19th February 2016) and will be presented as a poster at the annual congress of the European Association for the study of liver diseases [EASL (Barcelona, 13- 17th April 2016)]. This study is currently being writ- ten in the format of an article to be sent to a clinical journal indexed in international databases.
Finally, in 2015 an updating/remodelling process was started with the aim of extending the data col- lected in the database of this platform, at the same time as simplifying the registration of this data through its web page. This update/remodelling is currently still under way.
CIC BioGUNE.
Platform for Genomics, Proteomics, Metabolomics and Gene Silencing
Genomics Platform
Over 2015 the CIC BioGUNE platform for analysis of genomes carried out a total number of 40 services for characterisation of DNA or RNA from a total of 600 samples and a further 18 services or coopera- tion measures in projects for analysis of genotyping, transcriptomic, epigenomic and metagenomic data.
In this period the members of the group published 6 articles in journals of international impact on re- search done in cooperation projects, 2 chapters of a book and published a book entitled “Field Guidelines for Experi- mental Genetic Designs in High-Through- put Sequen- cing” which we consider will be a great help for any researcher wishing to include mass se- quencing technologies in their projects.
In mid-2015, the platform managed to join a spe- cial programme provided by the company Oxford Nanopore Technologies (The MinION Access Pro- gramme) to test its new mass sequencing system thanks to which we hope to be able to offer services connected with said technologies in the near future.
Metabolomics Platform
Over 2015, the metabolomics platform implement- ed the methodology of analysis by UPLC-MS of the metabolism of polyamines and glutathione, being incorporated to the already existing method for ana- lysing the metabolism of the methionine cycle. In 2015 these methods were used in the study of the disorders undergone by these metabolites during hepatic damage, and to evaluate the effectiveness of different treatments. Furthermore in 2015 new methods were developed to evaluate methionine adenosyl-transferase (MAT) activity as well as cat- echol-O-methyl transferase (COMT) activity in he- patic microsomes and samples of liver. Lastly, the preparation for carrying out fluoxomic studies was got under way. In 2015, the platform carried out 25 services and took part in three articles published in Frontier in Immunology, Journal of Hepatology and Cell Metabolism.
30 I Annual report 2015 I CIBEREHD