Page 73 - CIBERES-2015-eng

Most relevant scientific articles
Research Groups
Puig C., tiraDo-veLez J.M., CaLatayuD L., tuBau F., gar- MenDia J., arDanuy C. et aL. Molecular characterization of fluoroquinolone resistance in nontypeable haemophi- lus influenzae clinical isolates. Antimicrobial Agents and Chemotherapy. 2015;59(1):461-466.
earL C.s., Keong t.W., an s.-q., MurDoCh s., MCCarthy y., garMenDia J. et aL. Haemophilus influenzae responds to glucocorticoids used in asthma therapy by modulation of biofilm formation and antibiotic resistance. EMBO Molec- ular Medicine. 2015;7(8):1018-1033.
CuBero M., grau i., tuBau F., PaLLares r., DoMinguez M.a., Linares J. et aL. Hypervirulent Klebsiella pneumo- niae clones causing bacteraemia in adults in a teaching
Highlights
1. The pneumococcal clonal complex (CC) 156 is one of the major CC causing invasive pneumocccal disease. We analyzed the evolution of CC156 by whole genome sequencing throughout 25 years in our area. This is a new approach in the analysis of pneumococcal evolution that allow us to describe subclones with recombination events and antibiot- ic-multiresistance.
2. We described a novel typing method for S.pneu- moniae using selected surface proteins. After screening the allelic dispersion of 97 outer protein families in 19 complete pneumococcal genomes, 116 non-redundant surfotypes were identified. In or- der to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical iso- lates with different serogroup/serotype combina- tions were analysed. We found a significant corre- lation between surfotype and pathogenic behaviour (primary invasive, opportunistic invasive and non-in- vasive). (Collaboration with Group-3- CIBERes)
3. We analysed the frequency and the clinical and molecular epidemiology of Klebsiella pneumoniae bacteraemia isolates obtained over a 7-year period
hospital in Barcelona, Spain (2007-2013). Clinical Microbi- ology and Infection. 2015;:-.
aguinagaLDe L, Díez-Martínez r, yuste J, royo i, giL C, Lasa í et aL. Auranofin efficacy against MDR Strep- tococcus pneumoniae and Staphylococcus aureus in- fections.The Journal of antimicrobial chemotherapy. 2015;70(9):2608-17.
LLoBet e., Martínez-MoLiner v., Moranta D., DahLstroM K.M., regueiro v., toMasa a. et aL. Deciphering tissue-in- duced Klebsiella pneumoniae lipid a structure. Proceed- ings of the National Academy of Sciences of the United States of America. 2015;112(46):E6369-E6378.
(2007–2013) in HUB. Fifty-three of 878 Kpneumo- niae invasive isolates (5.4%) showed a hypermu- coviscous phenotype. Patients with magA+ and/or rmpA+ Klebsiella pneumoniae bacteraemia more frequently had pyogenic liver abscesses and pneu- monia than patients without hypermucoviscosity genes.
4. The use of in vitro and in vivo model systems al- lowed us to compare Azithromycin’s effects on infection by an Azithromycin-susceptible and an Azithromycin-resistant NT-H. influenzae clinical iso- late and to establish associations between Azithro- mycin efficacy, dose, bacterial MIC, and bactericidal and immunomodulatory properties.
5. Using a mouse model of infection, we show that corticosteroid treatment promotes H. influenzae persistence. The corticosteroid-responsive genes showed elevated expression in H.influenzae within sputum from patients undergoing steroid treat- ment. Addition of corticosteroid to H.influenzae led to alteration in biofilm formation and enhanced re- sistance to azithromycin.
Institution: Fundación IDIBELL · Contact: Hospital Universitario de Bellvitge · C/ Freixa Larga s/n. 08907 Hospitalet de Llobregat · Tel.: 93 260 79 30 · E.mail: [email protected]
CIBERES I Annual report 2015 I 73