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SEFALer
The “Servicio de fenotipado de animales de la- boratorio en red” (SEFALer) has the following ob- jectives: I) Functional and histological phenotyp- ing of animal models of human diseases; II) The archive of genetically modified mice; III) Continu- ous training; IV) Specialised and expert advice in phenotyping; and V) Scientific dissemination and disclosure.
The following achievements of SEFALer units are worthy of mention:
• I) Organisation of the 6th Sessions on Training in Phenotyping of Animal Models;
II) Participation in projects (FP7-AFHELO, FP7- TARGEAR and CDTI) for preclinical assess- ment of new drugs and development of cochle- ar implants for treating hearing loss.
Platforms
SEFALer has specifically offered the following range of services in 2015:
• Auditory, vestibular, respiratory and renal phenotyping, general pathological anato- my, neuro-behavioural (motor, cognitive and emotional), haematological and coagulation system, of demyelising diseases and motor coordination. As a result of this work publica- tions have been brought out with participation in research projects. Mention should be given to the COST-BM1402 MouseAGE action (http:// www.cost.eu/COST_Actions/bmbs/BM1402).
• Archiving and revitalisation of mutants in the Spanish node of EMMA/Infrafrontier (www.infrafrontier.eu).
SEFALer arranged the annual training course with Madrid’s Association of Veterinary Surgeons and the Facultad-Hospital Clínico Veterinario (UCM), with academic recognition and accreditation by the Continuous Training Commission of Health- care professions of the Madrid area. The educa- tional resources generated are available online. SEFALer units have also taken part in conferenc- es, congresses, seminars, scientific meetings and university education (UAM, UCM, UAB, ULL).
SEFALer also gave information and advice through [email protected] and http://www. ciberer.es/plataformas/sefaler, updating of links, information and news. It has an extensive network of national groups expert in phenotyping.
SEFALer took part in scientific dissemination ac- tivities (Researchers’ Night, Science Week, Brain Awareness Week...) and in sessions with patients’ associations.
• I) Giving services for pathological anatomy and phenotyping of the rental function, though without any significant increase in demand; II) Speaker at the 6th Sessions on Training in Phe- notyping Animal Models.
• I) Development of Behavioural phenotyping techniques with predictive value for transla- tional studies and on latest generation video- analysis techniques; II) Development of a sys- tem for analysis and display of Behavioural Big Data.
• I) Implementation of new protocols for the study of platelet function in flow conditions; II) Speaker at the 6th Sessions on Training in Phe- notyping Animal Models.
• Assessment of the locomotor phenotype (Treadmi- ll, Bar-cross, Clasping) and of axon- al (immunohistochemistry) in the model of X- linked adrenoleukodystrophy (double knockout Abcd1 and Abcd2 Abcd1-/Abcd2-/- mice) for Medday company, to test the MD1003 drug, with some very positive results. We go on with functional and molecular analysis of this prom- ising treatment for X-ALD.
• I) Implementation of ultra-superovulation tech- nology (Kumamoto University, Japan), which quintuplicates the production of oocytes, re- ducing the number of animals required for in vitro fertilisation/cryopreservation of embryos; II) Incorporation of Van Gieson and Sirius Red stains for detecting elastin and collagen fibres in histological preparations.
CIBERER I Annual report 2015 I 33